Composite

Part:BBa_K2201360:Design

Designed by: Maximilian Edich   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)


BBa_K2201260 downstream of the Carboxysome and T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 216
    Illegal NheI site found at 7224
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 374
    Illegal BamHI site found at 6667
    Illegal BamHI site found at 7348
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 882
    Illegal AgeI site found at 1833
    Illegal AgeI site found at 2514
    Illegal AgeI site found at 3417
    Illegal AgeI site found at 6058
    Illegal AgeI site found at 6170
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4566
    Illegal SapI site found at 274


Design Notes

It was considered to add also a T7 terminator at the end.


Source

Made of basic parts.

References